0000016709 00000 n Kouri T, Fogazzi G, Gant V, Hallander H, Hofmann W, Guder WG. ECLM. Dilution was also monitored with a range of specific gravity of 1.005–1.033 and a median result of 1.017. Similarly, protein reflectance data and total protein measurements correlated well (Spearman r = −0.921; P <0.001), which is expected as long as the major protein is albumin. The strips include reagent pads for ordinal scale reporting of relative density, pH, leukocyte esterase, nitrite, protein, glucose, ketones, urobilinogen, bilirubin, and hemoglobin/myoglobin. Access to the instrument’s raw data theoretically allows a higher analytical sensitivity for several analytes. Westgard JO. 3. We also wanted to explore the possibilities of combining the two novel methods, particularly in the analysis of erythrocytes, leukocytes, and glucose, which are of major clinical importance. Results: Reproducibility of the reflectance signal was good for high- and low-concentration urine pools for protein (0.8% and 0.9% and 1.5% and 2.2% within and between runs, respectively), leukocyte esterase (1.1% and 1.0%; 5.1% and 1.2%), hemoglobin (1.7% and 1.1%; 8.9% and 1.1%) and glucose (2.1% and 0.5%; 6.5% and 2.3%). SPERMIOGRAMMA. Langlois M, Delanghe J, Steyaert S, Everaert K, De Buyzere M. Automated flow cytometry compared with an automated dipstick reader for urinalysis. 1. Moreover, a 16% false-positive rate may be unacceptable for screening. >> Specific gravity (refractometry based) and clarity are measured in a flow cell, and color is rated with a specific algorithm against the blank pad on the test strip. 0000017558 00000 n Principio del test. The Sysmex UF-100 is a urinary flow cytometer-based walkaway instrument that performs automated microscopic analysis. ����,����bdm,�1"�Ql�m�[2ƥ+����M����}1��a4�Wrg��c�qE:�7輬M�-sx���L�+%{���^�" J�ك�M�Nm0�0�t���=Ƴ)׿1��?���L. When urinary WBC counts were higher than the upper reference limit (25 × 106 cells/L), the following regression equation was obtained: y (%) = 83.7 −15.4 logx (106 cells/L); Spearman r = −0.688; P <0.001. Anno: 2005 Modulo: 6 Identification of amyloidogenetic light chains requires the combination of serum-free light chain assay with immunofixation of serum and urine Conclusions: In urine test strip analysis, quantitative hemoglobin and leukocyte esterase reflectance data are complementary with flow cytometric results and glucose and albumin results. 0 Peele J, Gadsden R, Crews R. Semi-automated vs. visual reading of urinalysis dipsticks. bicchiere di plastica monouso). Examen de urina Pagina 8 - Reducere a capacitatii rinichilor de a asigura filtrarea si eliminarea produselor de rebut ale sangelui, de a controla echilibrul corpului in apa si saruri si de a regulariza presiunea sangvina. Agreement was fair between the flow cytometric RBC data (x) and the URISYS 2400 hemoglobin reflectance measurements (y) for counts above the upper reference limit (25 × 106 RBC/L on UF-100). (Insieme con il test viene fornito il contenitore urina da 90ml e un modulo in triplice copia).Questo Test Antidroga ha un sistema di stampa del risultato tramite il suo Strumento con software, oppure il risultato del test può essere anche scansionato o fotocopiato. Correlation between RBC counts (x; flow cytometry) and test strip hemoglobin concentration (y) for RBC counts >25 × 106 cells/L (n = 96). Hemoglobin reflectance (y; %) = 78.2 − 19.4 log(RBC count; 106 cells/L); (Spearman r = −0.680; P <0.001. http://www.westgard.com/lesson29.htm (Accessed May 2002).. Froom P, Bieganiec B, Ehrenrich Z, Barak M. Stability of common analytes in urine refrigerated for 24 h before automated analysis by test strips. ESAME URINE ESAME FISICO COLORE & ASPETTO DEL LIQUIDO LIMPIDO E OPALESCENTE (presenza di lipidi) TORBIDO (presenza di batteri sangue o pH alterato) Come si effettua ha perso di interesse ODORE DIVISO IN TRE PARTI si effettua tenendo in consideraione il mitto intermedio (si scarta Per informazioni e/o chiarimenti rivolgersi a : segreteria U.O. We also found good agreement between the hexokinase-based glucose method (x) and glucose test strip reflectance reading (y): y (%) = 53.9 − 19.3 logx (mmol/L); Spearman r = −0.851; P <0.001. Ottomano C. Tecnologia ed automazione dell’ esame urine quantitativo. Flow cytometry has been introduced for urinalysis (9)(10) to obtain quantitative data on urinary particles. No effect of conductivity on the leukocyte esterase field was noted. The protein test (n = 220) detected 86% (95% confidence interval, 78–92%) of samples with <30 mg/L albumin with a specificity of 84% (95% confidence interval, 76–91%). %PDF-1.4 Esame delle urine: si cambia Published in: La Rivista Italiana della Medicina di Laboratorio, July 2017 DOI: 10.1007/s13631-017-0157-7: Authors: Rudi Ravasio, Giulia Previtali, Francesca Nembrini, Maria Grazia Alessio View on publisher site Alert me about new mentions. Ce intelegem din rezultatul examenului de urina? Examenul de urina este un test de diagnostic si ajuta la identificarea substantelor si a materialului celular din urina, asociate cu diferite tulburari metabolice si renale. The reflectance value, expressed as a percentage within a range from 100% (white) to 0% (black), is therefore inversely related to the concentration of the analyte in the sample. trailer Of course, the frequency of erroneous results depends on the proportion of pathologic samples and on the preanalytical handling of samples. In conclusion, quantitative urine test strip analysis provides reliable data on WBCs, RBCs, glucose, and albumin. 0000017589 00000 n It could be of special interest in patients with undiagnosed diabetes or hypertension in whom microalbuminuria is regarded as an excellent marker for assessing early renal damage (4)(5)(6)(7), particularly because many patients with non-insulin-dependent diabetes mellitus are asymptomatic and their diabetic state remains undiagnosed for years (24). Because RBCs tend to lyse in urine, we investigated the linearity of the hemoglobin test strip pad. Recently, the URISYS 2400 automated urine test strip analyzer (Roche Diagnostics) was introduced. Test strip analysis plays an important role in urinalysis as such, and the value of test strip urinalysis as a screening method has been thoroughly demonstrated (1). Nulla è dovuto a pagamento dell’esame urine se l’interessato è minorenne o disabile : se maggiorenne, il pagamento di tale esame è compreso nell’importo dovuto per la visita. Istruzioni per esecuzione esame urine per idoneità sportiva agonistica Compilare il modulo sotto allegato necessario per l'accettazione del campione di urina . 2. 0000017717 00000 n Esame urine completo CHE MATERIALE SERVE PER LA RACCOLTA? Albumin in urine was measured immunonephelometrically on 220 randomly selected samples with use of commercially available Behring antibodies on a Behring Nephelometer II analyzer (Dade Behring) standardized against the widely accepted WHO/College of American Pathologists Certified Reference Material 470. Van Acker J, Verstraete A, Van Hamme M, Delanghe J. Falsely increased urinary caffeine attributable to contamination by urine test strips. Ultimo aggiornamento: 19 Luglio 2019 Condividi. 16 0 obj Day-to-day imprecision was assessed with control material: Liquichek Urinalysis Control Levels 1 and 2 (Bio-Rad). Because urinalysis test strips are often used for checking urinary flow cytometry data (11), there is a need for a more quantitative evaluation of urinalysis test strips. Because microalbuminuria is characterized by large intraindividual variability, an ordinal-scale answer might be misleading. Controlla la creatinina e Ph. Oxford University Press is a department of the University of Oxford. È un’analisi che valuta caratteristiche fisiche come colore, aspetto, odore, ma anche alcune componenti chimiche come proteine, sangue e glucosio. ATTENZIONE: Con questo servizio si accede direttamente alla sala prelievi (per prelievo sangue o tamponi + eventuale campione biologico).Per la consegna di solo materiale biologico (urine, feci, liquido seminale, etc..) è necessario utilizzare il servizio "SALTA LA … Whelan PV, McGill C, Ng K. Evaluation of the BMC Chemstrip urine analyzer and Chemstrip 10 UA test strip with emphasis on comparison with analytical, chemical, physical, and microscopic methods [Abstract]. 1. This instrument offers the possibility to obtain reflectance readings. As is the case for test strips in general, the method is based on the so-called “indicator error” principle, in which proton exchange between the indicator on the strip and the proteins in the solution produces a color change from yellow to green-blue. Search for other works by this author on: Author for correspondence. To fully explore the possibilities of the albumin test pad in first-line diagnosis, a formal study should be performed. Urina este un produs biologic care joacă un rol important în diagnosticul clinic, fiind obținută prin procedee neinvazive. COME RACCOGLIERE LE URINE? 0000029149 00000 n Alkalinization of urine gives rise to erroneous results in particle counting. Two discrepancies (0.9%) were found in which the test strips on both automated strip readers overestimated the urinary albumin concentration by >10-fold. • Inserire il codice esame (ECU ) e il codice richiedente 6650. This is stable for 30 days when stored tightly capped at 2–8 °C. Opis fizičkih i fizičko-hemijskih karakteristika mokraće: izgled, boja, miris, reakcija i specifična težina. Department of Clinical Chemistry University Hospital Ghent. The presence of haptoglobin in urine enhances the peroxidase activity of hemoglobin (29)(30). The inhibitory effect of urine on the peroxidase-like activity of hemoglobin and on horseradish peroxidase. Screening school children for albuminuria, proteinuria and occult blood with dipsticks. 14 22 Urine per esami di citologia urinaria raccolte Castelfranco Emilia. BREATH TEST. The two outer solid lines represent the 95% prediction interval around the regression line. Prendere nota dell'ora (ad esempio ore 7). Combining diagnostic information provided by urinary flow cytometry and more quantitative test strip analysis therefore offers a theoretical basis for the development of diagnostic expert systems (11)(12). 1. Mogensen C. Microalbuminuria predicts clinical proteinuria and early mortality in maturity-onset diabetes. This implies that the test has the potential to offer a screening for microalbuminuria without increased cost, hence coming closer to the “urine test strip of the future” (23). 0000016642 00000 n The lower limit of detection (18) was calculated as the mean value − 3 SD for a blank sample. Method validation-the detection limit experiment. 0000029025 00000 n The lower limit of detection was 25 mg/L (CV = 2.2%) (18). Nissell H, Kublickas M, Lunnell NO, Petterson E. Renal function in gravidas with chronic hypertension with and without superimposed preeclampsia. Methods: We compared URISYS 2400 (Roche) quantitative reflectance data with data from the UF-100 (Sysmex) and biochemical data for 436 nonpathologic and pathologic urine samples. Contenitore pulito (es. Test strip urinalysis was carried out before flow cytometry analysis (Sysmex UF-100; TOA Medical Electronics), using URISYS strips on a URISYS 2400 analyzer (Roche Diagnostics). Because of the procedure for applying the urine to the test strips on the URISYS 2400, improper dipping is no longer a problem, nor is confusion about sample identification or urine contamination caused by dipping the strip in the tube, which potentially leads to interferences with chromatographic methods (31). Particles that cannot be classified in one of the former categories are counted as “other cells”. The correlation coefficient for the glucose signal with the routine hexokinase-based method was −0.851. 2. <> Liquido seminale per citologia. The automation of sediment analysis using a new urine flow cytometer (UF-100). Of all the diagnostically relevant urinary markers, only albumin and transferrin accept protons well, so the potential error of missing Bence Jones proteinuria remains when test strip screening strategies are used in proteinuria (21), although many cases presenting with Bence Jones or tubular proteinuria show microalbuminuria (23). We found a close correlation between the log-transformed albumin results (x) and the test strip reflectance results (y): y (%) = 68.2 − 9.26[logx (mg/L)]; Spearman r = −0.825; P <0.001; Fig. 0000007901 00000 n In contrast, conductivity showed a t-value of only 1.477 (P = 0.141). Mattenheimer H, Adams EC. Richiesta di esame urine completo per idoneità sportiva agonistica (Cod.esenzione I01) PER ACCETTAZIONE e CONSEGNA CAMPIONE (Compilare IN STAMPATELLO il presente modulo con i dati dell’atleta) Cognome_____Nome_____ • Apporre il timbro del C. Prelievi presso cui è stato accettato il campione. Orsonneau JL, Douet P, Massoubre C, Lustenberger P, Bernard S. An improved pyrogallol red-molybdate method for determining total urinary protein. 0000000752 00000 n Fair agreement was obtained between UF-100 and test strip reflectance data for erythrocytes and hemoglobin (r = −0.680) and leukocytes and leukocyte esterase (r = −0.688). On the basis of ROC analysis, diagnostic sensitivity was 86% (95% confidence interval, 78–92%) and specificity was 84% (95% confidence interval, 76–91%) for a reflectance value of 55.6% when the nephelometric assay was used as the comparison method at a cutoff of 30 mg/L (n = 220; 113 positive and 107 negative cases, respectively). Because microalbuminuria is defined as excretion of 30–300 mg of albumin/24 h (20–200 μg/min, or 30–300 μg/mg of creatinine) (20), the protein field result allows identification of microalbuminuria cases in contrast to the classic reading and reporting of the strips, which can detect only albumin concentrations 150–200 mg/L or higher (21)(22). It has been extensively evaluated for urinalysis (9)(10)(11)(15) as well as for cerebrospinal fluid analysis (16). Articole Gratuite, Manopere - Recoltări probe microbiologice, Subiecte medicale. 0000016847 00000 n mikroskopski pregled taloga koji se … Correlation between protein (albumin) results obtained by quantitative test strip analysis (y) and albumin (x; Behring BN II Nephelometer; n = 220). Because microalbuminuria is generally regarded as an excellent marker for assessing early renal damage in common conditions such as diabetes and hypertension (4)(5)(6)(7) and as an early predictor of preeclampsia during pregnancy (8), the availability of highly sensitive test strip readers opens interesting perspectives for assessing this phenomenon. La documentazione di riferimento per l‟esecuzione dell‟esame urine dovrebbe comprendere una accurata descrizione della fase preanalitica, analitica e post-analitica. The ratio of hemoglobin reflectance to RBC count was constant when osmolality was >190 mosmol/L. Citologia per espettorato. Urine test strip analysis was performed with the automated URISYS 2400. Evaluation of the Sysmex UF-100 automated urinalysis analyzer. Scaderi anormale. 0000017492 00000 n S. C. Medicina di Laboratorio MODULO RACCOLTA URINE 24 ORE Revisione n.0 del 27/08/2017 PROCEDURE PER LA RACCOLTA DELLE URINE NELLE 24 ORE 1. The role of automated urine particle flow cytometry in clinical practice. Joris Penders, Tom Fiers, Joris R Delanghe, Quantitative Evaluation of Urinalysis Test Strips, Clinical Chemistry, Volume 48, Issue 12, 1 December 2002, Pages 2236–2241, https://doi.org/10.1093/clinchem/48.12.2236. It is known that reducing substances (e.g., ascorbic acid) may lower the signal, whereas oxidizing substances may have a positive effect on measured hemoglobin concentration. 059/21.34.280, nelle L'esame delle urine richiesto per la certificazione di idoneità sportiva agonistica deve essere effettuato prima della data della visita (entro i 30 giorni precedenti) utilizzando l'apposito modulo fornito dalla Società Sportiva per l'accesso al laboratorio analisi e seguendo le istruzioni. Modulo di richiesta: deve riportare Nome, Cognome, data di nascita e codice fiscale, struttura e/o Medico richiedente nonché i dati clinici essenziali (ematuria, litiasi, precedenti neoplasie vescicali, urine da catetere, da ureterostomia, da vescica ileale).. Modulo Esame Citologico. A lower limit of detection of 8 × 106 cells/L (CV = 1.2%) was calculated. Bacterial peroxidases can also contribute to total peroxidase activity in urine (23). The reproducibility of (semi)automated readings is at least as good as visual readings (2), but most authors find the analytical, clinical, and labor cost-saving advantages of (semi)automated vs visual reading to be obvious (3). questionario per l’esecuzione dell’esame citologico dell’urina (DA CONSEGNARE ASSIEME AI CAMPIONI RACCOLTI) QUESTO ESAME E’ STATO RICHIESTO DA: It therefore is advantageous to have a better estimation of the true concentration. ����~��ݟ_��3��?��s!b��c�x���S涴{���5�֓῰���ĭ#��B}��W������9���,��@Kw_9[g!�s�^�~m~=r��/�1ɸw�ֺ4�koK1��W!ň��8��DI99��� ��-t��,$н�3�_�X p� �Nd�zN���8�7����4�S�xS}�{Gi`E���s1k�� Bennett PH, Haffner S, Kasiske BL, Keane WF, Mogensen CE, Parving HH, et al. Urin je ultrafiltrat plazme in ga tvorijo ledvice. /Contents 17 0 R 4 depicts the correlation between both measurement methods and the lower limit of detection of 0.17 mmol/L (CV = 1.3%) with the 2 SD limits. The following regression equation was obtained: y (%) = 78.2 −19.4 logx (106 cells/L); Spearman r = −0.680; P <0.001; Fig. TAMPONI URETRALI. Modulo di prenotazione. 17 0 obj Regression equation for urinary WBC counts above the upper reference limit (25 × 106 cells/L; n = 132). In recent studies, the correlation (r) between UFC WBCs and WBCs by counting chamber was 0.93–0.98, and that of UFC RBCs and RBCs by counting chamber was 0.83–0.89 (10)(25)(26). Combur 10-Test M strips on a Miditron automated reflectance photometer (Roche) (13)(14), used in our routine laboratory, were used in parallel as a control. Does antihypertensive therapy protect the kidney in essential hypertension?. Background: Urine test strip results are generally reported in categories (i.e., ordinal scaled), but automated strip readers are now available that can report quantitative data. 3. Multiple regression analysis on the study population showed no effect of conductivity on test strip leukocyte esterase. This instrument offers th… Deferrari G, Repetto M, Calvi C, Ciabattoni M, Rossi C, Robaudo C. Diabetic nephropathy: from micro- to macroalbuminuria. Colombo JP eds. Test strip measurements had reasonable lower limits of detection for WBC (19 × 106/L). Tutela della salute nelle attività sportive, tel. Van Acker J, Delanghe J, Langlois M, Taes Y, De Buyzere M, Verstraete G. Automated flow cytometric analysis of cerebrospinal fluid. Urine per esami di citologia urinaria Sassuolo. Campione richiesto: urina fresca raccolta al mattino, dopo aver scartata quella della notte. The intensity of the reaction color of the test pad is detected by measuring the percentage of light reflected from the surface of the test pad. Diagnostic accuracy was assessed by ROC analysis using commercially available statistical software (MedCalc®). Barrare la sede nella quale si effettua la visita Via Alessandrini, 6 Consegnare il presente modulo e la provetta con le urine (almeno 4 giorni lavorativi prima della visita) presso uno dei seguenti Centri Prelievo nei giorni e negli orari indicati. In this study, the urinary hemoglobin concentration did not agree well with the RBC count obtained by flow cytometry. Fenili D, Pirovano B. La provetta contenente le prime urine della mattina deve essere portata e consegnata dall'utente o da suo? Pugia MJ, Lott JA, Kajima J, Saambe T, Sasaki M, Kuromoto K, et al. urine delle successive 24 ore, comprese quelle delle ore 7.00 del mattino successivo; durante tutto il periodo di raccolta mantenere il contenitore chiuso in luogo fresco e su superficie sicura, rigorosamente al buio e avvolto in carta stagnola, lontano dalla portata This offers several possibilities: (a) The sensitivity for albumin may allow affordable screening for microalbuminuria, particularly in patients with undiagnosed renal damage. The correlation between WBC data (x) and leukocyte esterase measurements (y) is shown in Fig. %%EOF Urine per esami di citologia urinaria Vignola. Examen sumar de urină Ce este examenul sumar de urină? 0000016924 00000 n Delanghe JR, Kouri TT, Huber AR, Hannemann-Pohl K, Guder WG, Lun A, et al. All samples were completely processed within 2–4 h after arrival. Screening and management of microalbuminuria in patients with diabetes mellitus: recommendations to the Scientific Advisory Board of the National Kidney Foundation from an ad hoc committee of the Council on Diabetes Mellitus of the National Kidney Foundation. Modulo di prenotazione. Raccogliere le prime urine del mattino o, in alternativa, le urine emesse dopo almeno 2 ore dalla prima. Title: Microsoft Word - Note informative Sito CdC Rizzola.doc Author: pichetti Created Date: 3/18/2020 4:11:07 PM 0000017284 00000 n Viene fornito il contenitore chiuso (capacità 3 litri) ed una provetta sottovuoto tappo bianco. o campione delle urine raccolte. 0000028490 00000 n In this study, we compared the URISYS 2400 automated strip reader with the Sysmex UF-100 flow cytometer to evaluate the possible value of quantitative test strip data in a urinary expert system. Recently, the URISYS 2400 automated urine test strip analyzer (Roche Diagnostics) was introduced. Fig. The two outer solid lines represent the 95% prediction interval around the regression line. Additional dilution experiments were carried out. The reproducibility of urinalysis using multiple reagent test strips. Od krvi se razlikuje po tem, da ne vsebuje krvnih celic (eritrocitov, levkocitov, trombocitov) in makromolekularnih snovi – beljakovin. Modificato il 18 aprile 2017 . P values <0.05 were considered significant. 0000016774 00000 n Glucose was measured by a hexokinase method standardized against Standard Reference Material 917a and Standard Reference Material 965 (n = 85), and total protein by a pyrogallol red method (17) (n = 129) with Standard Reference Material 917a as a standard and commercially available reagents for both measurement procedures (Roche) on a Modular P system (Roche). Predicting diabetic nephropathy [Letter]. Gambke B, Kouri T, Kutter D, Nagel D, Schmid R, Scholer A. Miditron® multicenter evaluation. The two outer solid lines represent the 95% prediction interval around the regression line. In our study correlating test strip data with urinary flow results, r was −0.69 between the flow cytometric WBC count and the leukocyte esterase reaction, although the presence of esterase inhibitors in urine and severe proteinuria might negatively affect test results for leukocyte esterase (1)(27).